Safety Evaluation of Seal Oil and its Lipid-Lowering Effect on Hypercholesterolemia Rats

Litao Yang • Yinlu Liu • Cuicui Bi • Yu Guo • Jian Zhao • Bo Zhang

Abstract: To study the toxicity of seal oil and its lipid-lowering effect on hypercholesterolemia, acute toxicity study, sub-acute toxicity study, genotoxicity studies and lipid-lowering test were done on mice or rats. The acute oral study was carried out in male and female Kunming mice. They took 19 mg/kg BW of seal oil orally for 14 days and observed and recorded. In the subacute oral toxicity study, rats were administered with three doses (0.83, 1.67, 3.33g/kg BW) by orally for 28 days. In genotoxicity studies, the genetic toxicity of Seal oil was tested through Ames test, in vivo mouse bone marrow micronucleus test and spermatocyte chromosomal aberration test. Safety of Seal oil was evaluated by acute oral toxicity study, subacute oral toxicity study and genotoxicity studies. Seal oil was not mutagenic and did not induce other chromosomal events. Additionally, seal oil was not genotoxic in Ames test, nor did it induce micronucleus of mouse bone marrow and chromosome aberration of spermatocytes in vivo test at the dose of 3.80g/kg BW. In the study of hypercholesterolemia rats, each dose of seal oil significantly reduced the cholesterol and triglycerides of hypercholesterolemia rats (P<0.01). Based on the results of the in vitro and in vivo studies, seal oil at a dose

of 19.00 g/kg BW did not show acute toxicity, and seal oil did not show sub-acute toxicity and genotoxicity. Seal oil can also reduce total cholesterol and triglyceride in hypercholesterolemia rats, which has a good lipid-lowering effect.

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